Oluwatayo Emmanuel Abioye*, Omotayo Olubunmi Ojo, Kazeem Adekunle Alayande, Olayinka Ayobami Ayegoro and David Ayinde Akinpelu

• access_time3 August, 2024

  subjectCategory: Biological Sciences

• Hibiscus sabdariffa calyx was assessed for nutritional value and biocidal potential. Proximate analysis revealed moisture content of 2.85%, while protein, fat and carbohydrate contents were 18.31%, 3.40% and 73.62% respectively. Phytochemical screening revealed presence of reducing sugars, flavonoids, steroids and cardiac glycosides. Inhibition zones due to sensitivity testing of the ethylacetate fraction (10 mg/ml) ranged between 10.33±0.58 and 17.17±0.76 mm against Pseudomonas aeruginosa(CISp1) and Escherichia coli (NCIB 86) respectively. The partially purified ethylacetate fraction (5 mg/mL) inhibition zones ranged between 12.00±1.00 (P. aeruginosa (CISp1)) and 24.00±0.00 (P. aeruginosa (NCIB 950)) while MIC ranged between 1.25 and 2.50 mg/mL. After 120 min of direct contact between the fraction (1×MIC) and Staphylococcus aureus, total cell death was 74.7%. Absolute mortality rate was achieved 3×MIC concentration at 120 mins and 60 mins for Staphylococcus aureus and Klebsiella pneumonia, respectively. Amount of K+ leakages at 1×MIC was 2.24μg/mL from S. aureus and 3.38μg/mL from K. pneumoniae. In general, leakages of cytoplasmic contents increase with increase in the concentration of the extract and the contact time. The green calyx of H. sabdariffa was found to have combined nutritional and medicinal values, thus, confirm its traditional application as food and in the treatment of infections.
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