Isolation And Identification Of Bacteria Associating To Necrotizing Fasciitis

Iloputaife, EJ.
  • access_time10 August, 2024

    subjectCategory: Biological Sciences

  • Bacterium(a) entrance into a host either through an external injury or by direct spread from a punctured/perforated internal organ (particularly the colon, rectum, or anus) or sexual organ are the means of contacting necrotizing fasciitis. This study was aimed at isolation and identification of bacteria that are associated to necrotizing fasciitis, to determine the spectrum of bacterial organisms responsible for necrotizing fasciitis. A total of twenty (20) samples were collected from patients with sterile swap sticks. Collected samples were cultured on Nutrient, MacConkey and Blood Agar plates and incubated at 37oC for 24 hours. Isolation and identification of bacteria was done using standard microbiological procedures. Results showed that Staphylococcus aureus was the commonest isolates accounting for 20(22%) followed by Escherichia coli 17(18.47%), Pseudomonas aeruginosa 16(17.6%), Streptococcus sp., 15(16.5%), Klebsiella sp. 13(14.2%) while Proteus sp. was the least common isolates accounting for only 10(11%). In conclusion, it is evident from this study that necrotizing fasciitis is largely polymicrobial, deriving significant contributions from both gram-positive and gram-negative organisms. It showed that wound samples used in this study were contaminated with bacteria and are therefore at risk of infection if proper care and treatment modalities were not adhered to. It is therefore a necessity that good hygiene and proper care of wound infection plus the cooperation of antimicrobial drugs during treatment.
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